http://dspace.cus.ac.in/jspui/handle/1/6400 2026-04-13T16:33:46Z http://dspace.cus.ac.in/jspui/handle/1/6407 Title: Cytogenetic and ITS-psbA-trnH sequence analysis for phylogenetic inference in Mucuna sp. of India Authors: Jaheer, M.; Chopra, R.; Kunder, K. R.; Bhat, Deepika; Rashmi, K. V.; Sathyanarayana, N. Abstract: This paper reports new chromosomal information and earliest proof on efficacy of nrITS and cp-psbA-trnH gene sequences for barcoding and phylogenetic studies in Mucuna sp. First cytological evidence on M. sempervirens and M. bracteata revealed symmetrical karyotype in both the species. Meiotic anomalies evident from the formation of chromosomal laggards and bridges in M. gigantea and M. atropurpurea and unreduced pollens in M. bracteata suggest evolutionary events that might have shaped annual species in this genus. Molecular investigations using nrITS and cp-psbA-trnH sequences revealed them to be phylogenetically informative. Vis-à-vis individual genes, combined sequences of ITS and psbA-trnH sequences offered reliable data for species delineation. The results summarized here are expected to galvanize molecular taxonomic studies and open-up newer means for answering phylogenetic questions in this genus. 2015-01-01T00:00:00Z http://dspace.cus.ac.in/jspui/handle/1/6406 Title: Role of genetic resources and molecular markers in Mucuna pruriens (L.) DC improvement Authors: Sathyanarayana, N.; Mahesh, S.; Leelambika, M.; Jaheer, M.; Chopra, R.; Rashmi, K. V Abstract: Mucuna pruriens (L.) DC is a tropical legume cover crop with promising nutritional and agronomic potentials. It is also a key source of 3,4 dihydroxy-L-phenylalanine (L-Dopa) – a precursor of dopamine used in the treatment of Parkinson's disease. However, lack of well-characterized germplasm plus poor accessibility to genomic resources has hindered its breeding programs. Furthermore, the cause and effect of various biotic and abiotic stresses impacting yield is also little studied. Systematic collection and evaluation of Indian germplasm by our group revealed presence of a diverse gene pool in India that can support a variety of breeding needs. The stability of L-Dopa trait across environments examined through Genotype and environment (G × E) interaction studies, as well as feasibility check on barcoding and phylogenetic analyses based on karyotype and conserved nuclear and chloroplast genes showed promising outcome. Germplasm screening for select biotic abiotic stresses identified resilient genotypes. Advances in use of DNA markers for diversity analysis, linkage map development, tagging of genes/quantitative trait loci for qualitative and quantitative traits, and progress in genomics are presented. 2016-01-01T00:00:00Z http://dspace.cus.ac.in/jspui/handle/1/6405 Title: A review on current status and future prospects of winged bean (Psophocarpus tetragonolobus) in tropical agriculture Authors: Lepcha, Patrush; Egan, Ashley N.; Doyle, Jeff J.; Sathyanarayana, N. Abstract: Winged bean, Psophocarpus tetragonolobus (L.) DC., is analogous to soybean in yield and nutritional quality, proving a valuable alternative to soybean in tropical regions of the world. The presence of anti-nutritional factors and high costs associated with indeterminate plant habit have been major concerns in this crop. But occurrence of good genetic variability in germplasm collections offers precious resources for winged bean breeding. However, lack of germplasm characterization is hindering such efforts. From a genomic standpoint, winged bean has been little studied despite rapid advancement in legume genomics in the last decade. Exploiting modern genomics/breeding approaches for genetic resource characterization and the breeding of early maturing, high yielding, determinate varieties which are disease resistant and free of anti-nutritional factors along with developing consumer friendly value-added products of local significance are great challenges and opportunities in the future that would boost cultivation of winged bean in the tropics. We review past efforts and future prospects towards winged bean improvement. 2017-01-01T00:00:00Z http://dspace.cus.ac.in/jspui/handle/1/6404 Title: Targeted metabolic and genomic profiling reveals parents for L-Dopa breeding in Mucuna pruriens (L.) DC Authors: Leelambika, M.; Mahesh, S.; Jaheer, M.; Tripathi, P. K; Kumar, P. Ranjith; Sathyanarayana, N. Abstract: Integrating targeted metabolic profiling with genetic diversity estimates is vital for selecting genetic stocks for breeding as well as mapping a biochemical trait. In Mucuna pruriens (L.) DC., copious amount of L-Dopa in seeds is viewed both as a boon and bane. Our objective of this work was to assess in conjunction both L-Dopa and genomic diversity in a selected set of germplasm to elucidate their relationship and identify parents for L-Dopa breeding/mapping. The findings revealed good genetic as well as metabolic (L-Dopa) diversity among the studied accessions. However, we could not establish direct relationship between these two as the marker data measured using AFLP and ISSR were not correlated with the seed L-Dopa contents. Based on the consensus information from both the data sets, seven parental combinations have been suggested. These findings are expected to pave way for genetic improvement as well as genetic mapping of L-Dopa trait besides integration of molecular markers in M. pruriens breeding programs. 2016-01-01T00:00:00Z